Calcineurin B availability for research

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Topic: Calcineurin B availability for research

Pan TAU
Member

posted 11-23-2003 10:29 PM

Calcineurin is a calcium binding and calmodulin binding protein found in all cells from yeast to mammals. It is a calcium dependent, calmodulin stimulated protein phosphatase regulating aspects of both cell wall synthesis and ion homeostasis in yeast cells. It also modulates the rate of pheromone-induced Ca2+ uptake and is required to maintain normal vacuolar morphology during prolonged pheromone treatment.
Calcineurin is discussed to be involved in myocardial infarction, stroke, alzheimer´s disease, renal diseases, potentation and memory.
Calcineurin B is a heterodimeric Ca2+/calmodulin-dependent protein phosphatase composed of two components (A and B). The A component is the catalytic subunit and the B component confers calcium sensitivity. Now this interesting enzyme is available from a commercial source in its recombinant human origin. The phosphatase is expressed in E. coli and equalls the native, full-length human CnB. The molecular weigth of CnB is 19.3 kDa. The purity of the delivered enzyme is >90% (by SDS-PAGE). Since it is a recombinant protein you don´t have to deal with potentially biohazard material of human origin.
With the availability of Calcineurin B in larger quantaties research groups can launch new approaches towards the investigation of functional ways of oxidative stress, neurodegeneration, stroke and cardiovascular diseases.
If you are interested please reply to this entry.

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