| June 13, 2010 |
|---|
| 9:00-9:30 am | Introduction
Welcome, Introductions, Define Course Goals
Watkins/Piston |
| 9:30-10:30 am | The Microscope 1
From Leuwenhoek to Digital Imaging
Watkins |
| 10:30-11:00 am | Break |
| 11:00-12:00 pm | The Microscope 2
Transmitted Light Contrast in Microscopy
Piston |
| 12:00-1:00 pm | Lunch |
| 1:00-2:30 pm | LAB
TRANSMITTED LIGHT MICROSCOPY |
| 2:30-3:00 pm | Break |
| 3:00-3:30 pm | The Microscope 3
Principal of Fluorescence
Piston |
| 3:30-4:30 pm | The Microscope 4
Fluorescence Microscope Design
Piston |
| 4:30-5:00 pm | Break |
| 5:00-5:30 pm | The Microscope 5
Optical Filters for Fluorescence Microscopy
Stanley |
| 5:30-6:00 pm | Digital Imaging 1
Digital Imaging and Detector Systems
Watkins |
| 6:00-7:00 pm | Dinner |
| 7:00-8:00 pm | LAB
SIGNAL-TO-NOISE IN DIGITAL IMAGING |
| 8:00-11:00 pm | LAB
STANDARD FLUORESCENCE MICROSCOPY |
| June 14, 2010 |
| 9:00-9:30 am | Digital Imaging 2
Resolution in Digital Imaging
Watkins |
| 9:30-10:00 am | Confocal 1
Introduction to Confocal Microscopes
St. Croix |
| 10:00-10:30 am | Confocal 2
Confocal Detectors and "Pseudo-confocals"
Piston |
| 10:30-11:00 pm | Break |
| 11:00-12:00 am | Live Cell Imaging 1
TIRF, Structured Illumination
Kenworthy |
| 12:00-1:00 pm | Lunch |
| 1:00-4:30 pm | LAB
CONFOCAL MICROSCOPY |
| 4:30-5:30 pm | Tips and Tricks
Practical Digital Imaging
Watkins |
| 6:00-7:00 pm | Dinner |
| 8:00-11:00 pm | LAB
IMAGE PROCESSING & FEATURE EXTRACTION |
| June 15, 2010 |
| 9:00-9:30 am | Image Processing 1
Digital Filters
Watkins |
| 9:30-10:30 am | Image Processing 2
Deconvolution
Piston |
| 10:30-11:00 am | Break |
| 11:00-12:00 pm | Confocal 3
3D Methods, Microfluorimetry Applications
St. Croix |
| 12:00-1:00 pm | Lunch |
| 1:00pm-4:30 pm | LAB
MULTICOLOR 3-D IMAGING |
| 4:30-5:30 pm | Tips and Tricks
Cell Labeling, Antibodies, GFP, Practical Aspects
Watkins |
| 6:00-7:00 pm | Dinner
Evening Off |
| June 16, 2010 |
| 9:00-10:30 am | Live Cell Imaging 2
Advanced Imaging Techniques, Quantitation
Kenworthy |
| 10:30-11:00 am | Break |
| 11:00 am - 12:00 pm | Multiphoton 1
Theory, Instrumentation, Applications
Piston |
| 12:00 noon | Course Photo |
| 12:00-1:00 pm | Lunch |
| 1:00-4:30 pm | LAB
DECONVOLUTION, APOTOME, FRAP |
| 4:30-5:30 pm | Tips and Tricks
Live Cell Imaging Microscope Design
Watkins |
| 6:00-7:00 pm | Dinner |
| 7:00-8:00 pm | LAB
MULTIPHOTON, TIRF LAB |
| June 17, 2010 |
| 9:00-10:30 am | Live Cell Imaging 3
Multicolor and Ratio Imaging
St. Croix |
| 10:30-11:00 pm | Break |
| 11:00-12:00 pm | Live Cell Imaging 4
FRET and Live Cell Imaging
Kenworthy |
| 12:00-1:00 pm | Lunch |
| 1:00-4:00 pm | LAB
LIVE CELL FRET IMAGING |
| 4:00-4:30 pm | LAB
LIVE CELL CHAMBERS, SETUP COLLABORATIVE GROUPS BASED ON STUDENT INTERESTS |
| 4:30-5:30 pm | Tips and Tricks
How to Manage Data, Make Movies, Etc
Staff |
| 6:00-7:00 pm | Dinner |
| 7:00-11:00 pm | LAB
RATIO IMAGING METHODS TRACKING, QUANTITATION, STUDENT EXPERIMENTS |
| June 18, 2010 |
| 9:00-10:00 am | Live Cell Imaging 5
Comparison of Optical Sectioning Approaches
Piston |
| 10:00-12:00 am | LAB
COLLECT DATA, PREPARE PRESENTATIONS |
| 12:00-2:00 pm | Lunch |
| 2:00-4:00 pm | Results!
Student Presentations |
| 4:00-4:30 pm | Mechanics
How Do I Get Money to Pay for All This Stuff?
Watkins |
| 4:30-5:00 pm | Summing up
Q&A, Student Feedback
Faculty |
| 5:30 pm | Lobster Bake
Caravan to Thompson Island Picnic Area |