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Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory
July 21 - August 10, 2009
Advances in light microscopy, digital image processing, and the development of a variety of powerful fluorescent probes present expanding opportunities for investigating the nervous system, from synaptic spines to networks in the brain. This intensive laboratory and lecture course will provide participants with the theoretical and practical knowledge to utilize emerging imaging technologies. The primary emphasis of the course will be on vital light microscopy. Students will learn the principles of light microscopy, as well as use of different types of electronic cameras, laser scanning systems, functional fluorophores, delivery techniques, and digital image-processing software. In addition to transmitted light microscopy for viewing cellular motility, the course will examine a variety of molecular probes of cell function, including calcium-sensitive dyes, voltage-sensitive dyes, photo-activated ("caged") compounds, and exocytosis tracers. Issues arising in the combination of imaging with electrophysiological methods will be covered. Particular weight will be given to multi-photon laser scanning microscopy and to newly available biological fluorophores, especially Green-Fluorescent Protein (GFP) and its variants. We will use a spectrum of neural and cell biological systems, including living animals, brain slices, and cultured cells.
Applicants should have a strong background in the neurosciences or in cell biology. In their personal statements, applicants should specify: 1) their experience with optical techniques, 2) how they will apply optical methods in their current projects, 3) the microscope systems available to them, and 4) their long-term goals in learning more about optical methods.Experience with basic recombinant DNA techniques is a prerequisite for admission to this course. Lectures by the instructors will cover the current status of the gene expression field, theoretical aspects of the methodology, and broader issues regarding strategies for investigating the regulation of gene expression in eukaryotes. Guest lecturers will discuss contemporary problems in eukaryotic gene regulation and technical approaches to their solution.
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Organized by:
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Scott Thompson, University of Maryland William Tyler, Arizona State University Jack Waters, Northwestern University |
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Invited Speakers:
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2009 Speakers to be announced Lecturers in the 2008 course included:
Hemant Bokil, Cold Spring Harbor Laboratory
Marcel Bruchez, Carnegie Mellon University
Karl Deisseroth, Stanford University
Winfried Denk, Max-Planck-Institute for Medial Research
David DiGregorio, Universite Paris 5
Florian Engert, Harvard University
Oliver Griesbeck, Max Planck Institute of Neurobiology
Samuel Hess, University of Maine
Mark Huebener, Max-Planck-Institute of Neurobiology
David Kleinfeld, UCSD
Jurgen Klingauf, Max Planck Institute for Biophysical Chemistry
Fred Lanni, Carnegie-Mellon University
Jeff Lichtman, Harvard University
Troy Margrie, University College of London
Partha Mitra, Cold Spring Harbor Laboratory
Thomas Mrsic-Flogel, University College London
Venkatesh Murthy, Harvard University
Valentin Naegerl, Max-Planck-Institute of Neurobiology
Timothy Ragan, Massachusetts Institute of Technology
Karel Svoboda, HHMI
Philbert Tsai, University of California, San Diego
Richard Weinberg, University of North Carolina
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Deadline for Abstracts:
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Application Deadline March 15
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Registration:
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Online Application Information
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E-mail:
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meetings@cshl.edu
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