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Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory
July 17 - August 1, 2009
This intensive laboratory and lecture course will focus on cutting-edge proteomics approaches and technologies. In the protein profiling portion of the course, students will gain hands-on experience in several quantitative proteome analysis methods, including two-dimensional gel electrophoresis and isotopic labeling strategies. Students will be trained to use DIGE, or differential in-gel electrophoresis, for gel-based protein quantification. Differentially expressed proteins will be determined using advanced gel analysis software, and identified using MALDI mass spectrometry. Students will be taught differential and quantitative mass spectrometry approaches to profile and identify changes in proteomes. Students will be trained in high-sensitivity microcapillary liquid chromatography coupled with nanospray-ESI and tandem mass spectrometry. Both single dimension and multidimensional microcapillary liquid chromatography separations coupled to mass spectrometry will be taught. Students will gain hands-on experience purifying and identifying protein complexes and post-translational modifications using the latest technologies. A strong emphasis will be placed on data analysis throughout the course. The overall aim of the course is to provide each student with the fundamental knowledge and hands-on experience necessary to be able to perform and analyze proteomics experiments, and to learn to identify new opportunities in applying proteomics approaches to his/her own research.
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Organized by:
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Tracy Andacht, University of Georgia James E. Bruce, University of Washington Andrew Link, Vanderbilt University School of Medicine Darryl Pappin, Cold Spring Harbor Laboratory |
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Invited Speakers:
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2009 Speakers to be announced Speakers in the 2008 course included:
Roger Biringer, Life Sciences Mass Spectrometry
Steven Carr, Broad Institute of Harvard and MIT
Brian Chait, Rockefeller University
Pierre Chaurand,Vanderbilt University School of Medicine
Karl Clauser, Broad Institute of Harvard & MIT
Ileana Cristea, Princeton University
Paul Huang, MIT
Darryl Pappin, Applied Biosystems (now CSHL)
Michael Snyder, Yale University
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Deadline for Abstracts:
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Application Deadline March 15
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Registration:
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Online Application Information
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E-mail:
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meetings@cshl.edu
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