Ion channel Physiology

Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory
June 3-23, 2009

The Advanced Bacterial Genetics course presents logic and methods used in the genetic dissection of complex biological processes in diverse bacteria. Laboratory methods include: classical mutagenesis using transposons, mutator strains, and chemical and physical mutagens; detection and quantitation of gene expression changes using various reporter genes and real-time PCR; the mapping of mutations using genetic and physical techniques; modern approaches to the generation and analysis of targeted gene disruptions and fusions using PCR and cloning methods; epitope insertion mutagenesis; site-directed mutagenesis; and fluorescence microscopy. Key components of the course will be the use of sophisticated genetic methods in the analysis of model bacteria (including E. coli, Salmonella, Bacillus subtilis, and Myxococcus xanthus), and the use of the wealth of new genomic sequence information to motivate these methods.

Invited lecturers will present various genetic approaches to study bacterial mechanisms of metabolism, development, and pathogenesis.

To be announced

Guest speakers in 2008 included:
Francisco Bezanilla,The University of Chicago
Joshua Dudman, Janelia Farm Research Campus
Nace Golding, The University of Texas at Austin
Dax Hoffman, NIH/NICHD/LCSN
Jeffry Isaacson, UCSD
Amy Lee Emory, University School of Medicine
David Linden, Johns Hopkins University School of Medicine
Eve Marder, Brandeis University
Andreas Schaefer,Max-Planck-Institut f. medizinische Forschung
Jesper Sjostrom, University College London
Ivan Soltesz, University of California, Irvine
Nelson Spruston, Northwestern University