Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory
April 17-28, 2009
Xenopus is the leading vertebrate model for the study of gene function in development. The combination of lineage analysis, gene-knockout strategies, experimental manipulation of the embryo, and genomic/bioinformatic techniques, makes it ideal for studies on the molecular control of embryo patterning, morphogenesis and organogenesis. The course combines intensive laboratory training with daily lectures from recognized experts in the field. Students will learn both emerging technologies and classical techniques to study gene function in Xenopus development. An important element will be the informal interaction between students and course faculty. Technologies to be covered will include: oocyte and embryo culture, lineage analysis and experimental manipulation of embryos, time lapse imaging of morphogenesis, gain and loss of function analysis using mRNAs and antisense oligos, whole mount in situ hybridization, immunocytochemistry, RT-PCR, and genomic/bioinformatic techniques, preparation of transgenic embryos, and use of Xenopus tropicalis for genetic analyses. The Cell and Developmental Biology of Xenopus course is designed for those new to the Xenopus field, as well as for those wanting a refresher course in the emerging technologies. The course is open to investigators from all countries.
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Invited Speakers:
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To be announced Lecturers and assistants for the 2008 included:
Ken Cho, University of California, Irvine
Jan Christian, Oregon Health and Sciences University
Rob Grainger, University of Virginia
Richard Harland, University of California, Berkeley
Janet Heasman, Cincinnati Children's Hospital Res Foundation
Mustafa Khokha, Yale University School of Medicine
Paul Krieg, University of Arizona College of Medicine
Takuya Nakayama, University of Virginia
Christopher Wylie, Cincinnati Children's Hospital Res Foundation
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