HUM-MOLGEN archive: BIOT: various

New BIOTechnology and Molecular Biology related mails on an amazing variety of subjects! Please find out whether there's a mail of your interest, and explore possible collaborations and opportunities! Please send high quality messages only; others will be refused without further notification. Please respond by private E-mail only. Arthur Bergen ********************************************************************** This message was originally submitted by ealcivar@GU.PRO.EC to the HUM-MOLGEN list at NIC.SURFNET.NL. My name is Irina Moleon Borodowsky. I am cuban biologist. I used to live in Cuba and I used to work in Medicine Tropical institute from Havana. Actually I live in guayaquil, Ecuador. I worked in PCR and hybridization techniques for some infectious pathogens. In this country, I am trying to fing the way appyling molecular biology in private diagnosis because my economical situation is very bad, and also I like of the molecular biology. Please if someone help me in tell me: which the best primers for the diagnosis of clamidia, what is the secuences, and what is biologic fluid that i may use it, the same for the papilloma virus, what is the best primers for use in pathology?-EPV, and fibrosis quistica I would like also to know if can to do a training in situ amplification for infectious agents and p53 I would appreciate any help. Thanks in advances You can contact with me to this e-mai: lealcivar@gu.pro.ec Irina Moleon Borodowsky ****************************************************************** This message was originally submitted by TAHMASEB@SABA.TMU.AC.IR to the HUM-MOLGEN list at NIC.SURFNET.NL. Dear Collegues, I would be very appretiated if anybody tell me how I can retieve the sequence of intron 19 of human F VIII gene by internet network. Yours Mohammad Tahmaseb tahmaseb@saba.tmu.ac.ir *********************************************************************** >From jcampusa@genes.bio.puc.clFri Oct 11 16:24:58 1996 jcampusa@genes.bio.puc.cl (Jorge Campusano) sent the following comments: ------------------------------------------------------------ I am a chilean biochemist student who is working in in situ hybridization of metabotropic glutamate receptors in rat brain slices. My problem is that i have problems with this technique and i really appreciate to comunicate with an investigator with who i could talk about my problems. ------------------------------------------------------------ Server protocol: HTTP/1.0 Remote host: Remote IP address: 146.155.211.48 ************************************************************************** Mgoral@bmclib2.bhs.org (Nick Markey) sent the following comments: ------------------------------------------------------------ I am looking for information on where to obtain the probe PW71 (D15S63 to use in methylation studies for identification of uniparental disomies of chromosome 15. Can anyone help me? ------------------------------------------------------------ Server protocol: HTTP/1.0 Remote host: library.bhs.org Remote IP address: 199.92.147.2 ************************************************************************** Dear colleagues, I am looking for information about acrocentric chromosomes. I am interested in the special staining properties of the different DNA-types in the short arms of these chromosomes; e.g. r-DNA>>>GC-rich>>>QFQ-/R-bands+/AgNOR+ does someone know the exact staining behaviour of 1. alpha-satellite DNA 2. beta-satellite DNA 3. satellite III DNA 4. the cytological satellite 5. rDNA If someone can answer me my questions please contact me. It would be very helpful for me! Thanks a lot! Markus Stumm ------------------------------------------------------------ Server protocol: HTTP/1.0 Remote host: mus.URZ.Uni-Magdeburg.DE Remote IP address: 141.44.11.10 ***************************************************************** Hi I am M.Sc student in Genetics.My thesis is about determination of allelic freqencies of restriction site polymorphisms on this gene.RFLP is the easiest way to carreier identification.allelic ferqencies of Hind III and BclI were easily determined by PCR(Polymerase Chain Reaction)but it seems dificult for XbaI polymorphism.It usually has unspecefic bonds in agarose gel electrophoresis . I would very appritiated if someboby tell me what the good condition is for obtatining good bands by PCR. Yours Sincerely Mohammad Tahmaseb tahmaseb@saba.tmu.ac.ir This message was originally submitted by ANDREA@MVCCH2.UNICA.IT to the HUM-MOLGEN list at NIC.SURFNET.NL. Dear sirs, I am a new graduated student of Chemistry who's looking for the improvement of his knowledges in the field of Degradation of recalcitrant tossic substances by new biotechnologies. I would like to receive any comment, suggestion or else from anyone more expert than me and, possibly, with the will of sharing impression and solving problems together. If you should have any suggestion to fit my request, please send it by e-mail to "Andrea@mvcch2.unica.it". From the elementary books to the newest article recently appeared in the specialized rewiews, up to the recent results on the trend of the market in the field, all will be helpful. Thanks a lot by now. Andrea Fera ***************************************************************************** >From GAMBACORTI@ICIL64.CILEA.ITFri Oct 11 16:26:09 1996 Date: Wed, 18 Sep 1996 10:22:42 +0000 > >ikr@ikr.nl (J.A.Groenink,MD) sent the following comments: > >------------------------------------------------------------ >Sirs, >Is there a simple PCR-based test available for the chimeric oncogen BCR-ABL ?Purpose is the the finding of minimal quantities after treatment for CML. > >J.A.Groenink,MD >Comprehensive Cancercentre Rotterdam, Holland >------------------------------------------------------------ >Server protocol: HTTP/1.0 >Remote host: poindexter.wirehub.nl >Remote IP address: 194.165.93.194 > **************************************************************************** From: "J.A.Groenink,MD" <ikr@ikr.nl> To: Multiple recipients of list ED-MOLGEN <ED-MOLGEN@NIC.SURFNET.NL> Subject: CML & PCR ikr@ikr.nl (J.A.Groenink,MD) sent the following comments: ------------------------------------------------------------ Sirs, Is there a simple PCR-based test availible to detect small quantities of of the chimeric oncogen BCR-ABL ? Purpose is the detection after treatment for CML. J.A.Groenink,MD Comprehensive Cancercentre Rotterdam, Holland ------------------------------------------------------------ Server protocol: HTTP/1.0 Remote host: poindexter.wirehub.nl Remote IP address: 194.165.93.194 ************************************************************************* >From panai@logos.cy.net This message was originally submitted by panai@LOGOS.CY.NET to the HUM-MOLGEN list at NIC.SURFNET.NL. I don't have a chromosome 22 specific library but I have a copy of the PAC library (Ioannou et al, Nature Genetics 6 (1994) 84-89 and unpublished work) (total genomic, average insert size ~120kb, 3x redundancy) which we can screen for you by PCR or filter hybridization on a collaborative basis, as long as you can cover our basic screening costs. The properties of this library make it ideal for recovering most genes intact with their regulatory sequences. Best regards, Panos Ioannou Panos Ioannou, PhD Coordinator, Dept. of Molecular Genetics The Cyprus Institute of Neurology & Genetics 6 International Airport Av. P.O. Box 3462, Nicosia 1683, Cyprus Tel: (357) 2 392652 (dir)/392650 (secr) Fax: (357) 2 358237/8 e-mail: panai@zenon.logos.cy.net (active until Sept. 96) e-mail: panai@mdrtc.cing.ac.cy (active from July 1 1996) ************************************************************** > Reply-to: R.deGroot@hli.azu.nl > From: "Rolf P. de Groot" <R.deGroot@hli.azu.nl> > Subject: Question > > Dear everybody > > We are trying to clone the promoter of a gene located on human > chromosome 22 for some time now. However, we are still unsuccessfull. > I wonder whether anybody could help us with a chromosome 22 specific > genomic library (phage or cosmid). We would be happy to cooperate > with anybody who could supply us with such a library. > > Thanks, > > Rolf P. de Groot > Rolf P. de Groot Tel: (31)30 2507134 / 6754 > Dept. Pulmonary Diseases Fax: (31)30 2542155 > Room G03.550 Email: R.deGroot@hli.azu.nl > University Hospital Utrecht Email2: pvlangen@worldaccess.nl > PO Box 85500 > 3508 GA Utrecht > The Netherlands ************************************************************************ >From luizmau@USP.BRFri Oct 11 16:26:33 1996 Subject: Re: BIOT: various August 1996 This message was originally submitted by luizmau@USP.BR to the HUM-MOLGEN list at NIC.SURFNET.NL. Would someone help me? I neek to extract DNa from plasma that have lost the buffy coat. Can I have some chance to get some DNA? I hava about 5 ml of plasma from each one. Mauricio %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% % Luiz Mauricio da Silva - luizmau@usp.br % % Departamento de Genetica FMRP - USP % %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% ******************************************************************************