Activating Genes In Live Animals

To understand the function of genes, it is advantageous to induce their expression in a live organism while controlling the spatial and temporal aspects of expression. With this in mind, Sidney Cambridge and colleagues modified a system known to work in cell culture so that it also worked within organisms.

The authors took small molecules known to activate genes adjacent to a particular promoter and inactivated these molecules by linking them to UV light-sensitive chemical compounds -- a process known as caging. Exposing cells containing caged molecules to innocuous levels of UV light removes the cage and allows activation of the gene regulated by the molecule-sensitive promoter. By precisely timing and localizing the activating light, the authors induced gene expression in single cells as well as whole organisms of mouse embryos and tadpoles.

Author contact

Sidney Cambridge (University of Heidelberg, Germany)
E-mail: cambridge@ana.uni-heidelberg.de

Abstract available online.

(C) Nature Methods press release.

Message posted by: Trevor M. D'Souza