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Advances in cell culture technology

 
  January 14, 2010  
     
 
The Biopark, Broadwater Road, Welwyn Garden City, Herts, AL7 3AX
15/04/10


9:00 – 9:45            Registration

 

9:45 – 10:00         Introduction by the Chair:  Professor Stefan Przyborski, School of Biological and Biomedical Science, Durham University, UK

 

10:00 – 10:30       Cell line misidentification
Professor John Masters, UCL, UK
The failure to identify the species and individual from which a cell line originated has resulted in the use of misidentified cell lines and an enormous financial cost to the academic and pharmaceutical research community. Simple, cheap and reproducible quality control measures are available to distinguish species and to identify individual human, cow, horse and dog DNA. Currently, the ATCC is developing a standard for cell line identification which will be adopted as an American standard.

                               

10:30 – 11:00       Recent advances in cell culture methods as alternatives to animal testing
Dr Carol Barker, 
Core Technology Facility, UK
The talk will summarise recent developments in cell culture methods as valuable alternatives to animal testing, and highlight some of the remaining key challenges.  The main focus will be on human cell culture systems currently available for cosmetic testing and drug discovery applications, and how these systems can assist companies to comply with the 7th Amendment to the European Cosmetics Directive and REACH legislation

 

11:00 – 11:10       Speakers photo

11:10 – 11:30       Mid-morning break

 

11:30 – 12:00       Metabolomic profiling of mammalian cell lines – New approaches for understanding recombinant antibody production

                                Dr Christopher A. Sellick , University of Manchester, UK

                                Mammalian cells (e.g. CHO or NS0 cells) are very important industrially for production of biopharmaceuticals due to the requirement for mammalian-type post-translational processing and appropriate folding. Metabolomics is proving to be a powerful tool for understanding responses of these cells to different medium formulations with and without the addition of feeds. Combined analysis of the fingerprint and footprint of cells under different growth conditions has enabled us to define key limiting metabolites that have been used to define simple rational feeds (containing only 5 metabolites) that improve antibody production by 100%.

 

12:00 – 12:30       Development of Technology for the Routine Growth of Cells in Three Dimensions

                                Professor Stefan Przyborski Director and Chief Scientific Officer, ReInnervate Limited, UK

 

12:30 –14:00        Lunch and Poster Viewing

 

14:00 – 14:30       Harvest your cells without trypsin! Introducing cell releasing UpCell surface by temperature control

Dr  Masato Ishiwata, CellSeed Inc, Japan

UpCell culture surface regulates cell adhesion and detachment by mere temperature control. UpCell surface is covalently bonded with temperature-responsive polymer and is slightly hydrophobic at 37C allowing cell adhesion and proliferation. However the surface becomes extreme hydrophilic under 32C, prompting cell detachment without cell damaging proteolytic enzymes such as trypsin. Applications include immunobloting and flow cytometry, detachment of strong adherent macrophage or osteoclast. When cells are cultured beyond over confluence, the contiguous and scaffold free cell-sheet can be collected with deposited extra cellular matrix which can be used for regenerative medicine study such as cornea or cardiovascular indication.

 

14:30 – 15:00       Embryonic stem cell bioprocessing
Dr Farlan Veraitch, UCL, UK

 

15:00 – 15:30      Instant immortal cell cultures from Cdkn2a null mice: functional analysis of colour mutations using melanocyte lines
Professor Dorothy C Bennett, Molecular and Metabolic Signalling Centre, Division of Basic Medical Sciences, St George's, University of London, UK

Normal somatic cells can undergo a limited number of divisions before undergoing the permanent growth arrest called cell senescence.  Although an important tumor suppressor mechanism, senescence is an inconvenience for cell culturists.  A key player is the Cdkn2a locus, encoding two powerful growth inhibitors and mediators of cell senescence, p16 and ARF.  From mice with deletions at this locus, one can culture quite normal, functional cells, such as pigmented melanocytes, which do not senesce: they are immortal immediately.  We have utilized this finding to study numerous pigmentary mutations in immortal cultured melanocytes, working with many other groups worldwide.

                               

15:30– 16:00       Afternoon Tea/Coffee and Last Poster Viewing

16:00 – 16:30      Wnt signals in Prostate Cancer and Embryonal Carcinoma Cell Growth and Differentiation

Dr Robert Kypta, Center for Cooperative Research in Biosciences (CIC bioGUNE, Spain and Imperial College London, UK

                Wnt signals play important roles in cell growth and differentiation and are aberrantly activated in many tumours. Our goal is to understand how Wnts, their antagonists and their effectors control cell growth and differentiation. We study these aspects in two contexts – prostate cancer (PCa) progression and neural differentiation. In addition, we are studing the differingentiation of cancer and stem cells cultured in microgravity bioreactors.

 

16:30 - 17:00       Developing in vitro 3d models of breast cancer

Dr Debbie Holliday, Department of Pathology and Tumour Biology, Leeds Institute of Molecular Medicine, UK

A major challenge in studying cancer in vitro is the lack of relevant model systems which accurately represent human disease. This talk will describe the development and characterisation of a multi-cellular organotypic model of pre invasive breast cancer.  It will demonstrate that these models can be used to study cellular function and also as a potential tool to screen new drugs of interest.

17:00                      Chairman’s summing up

 

 

 
 
Organized by: Euroscicon
Invited Speakers:

Chair: Dr Stefan Przyborski, School of Biological and Biomedical Science, Durham University, UK

Professor John Masters, UCL, UK

Dr Carol Barker, Core Technology Facility, UK

Dr Christopher A. Sellick , University of Manchester, UK

Dr  Masato Ishiwata, CellSeed Inc, Japan

Dr Farlan Veraitch, UCL, UK

Dr Robert Kypta, Center for Cooperative Research in Biosciences (CIC bioGUNE, Spain and Imperial College London, UK

Dr Debbie Holliday, Department of Pathology and Tumour Biology, Leeds Institute of Molecular Medicine, UK
 
Deadline for Abstracts: The Deadline for abstract submissions for oral presentation is February 10th 2010
 
Registration:  

The Deadline for early registration is February 20th 2010

After this time the fees double, so make sure you register early!

Before the early registration deadline

After the early registration deadline

Standard

 £249 + VAT

 £485 + VAT 

Academic

 £149 + VAT

 £298 + VAT

Student

£99 + VAT

£198 + VAT

IBMS

 £149 + VAT

 £298 + VAT

E-mail: astrid.englezou@euroscicon.com
 
   
 
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