The Knowlede Foundation, Atlanta, GA
June 14-15, 2007
Thursday, June 14, 20078:00 Registration, Exhibit/Poster Setup, Coffee and Pastries Sensing/Detection/Identification and Emerging Detection Technologies: A Top-Down View 8:45 Biothreat Detection and Diagnosis: The Joint Biological Agent Identification and Diagnostic System (JBAIDS) - Current Status and Future Vision
James W. Karaszkiewicz, PhD, Senior Scientist, Goldbelt Raven LLC; Chief Scientist, JBAIDS Program Office, Medical Identification and Treatment Systems Joint Product Management Office; and
David H.-F. Teng, PhD, Director of Biochemistry, Idaho Technology, Inc.
The Joint Biological Agent Identification and Diagnostic System (JBAIDS) is the U.S. Department of Defense program of record for the detection and diagnosis of biothreat agents. JBAIDS provides a standardized, validated and FDA-cleared, multi-service solution that is ruggedized, deployable, and provides demonstrated capability for pathogen identification in both environmental and clinical sample types. This presentation will describe the current JBAIDS technology and discuss a vision for the future. 9:15 Requirements of Biosensor Technologies by Municipal Water Laboratories: Pathogens of Interest and Hardware Requirements
Tammy A. Spain, PhD, Senior Chemist/Technical Director of Microbiology, Analytical Division, Pinellas County Utilities Laboratory
Most of the biosensor technologies available on the market have the potential to test water for pathogens. Yet municipal water systems have been extremely challenging markets for biosensors. This presentation will provide an overview of biosensor technologies that might be useful to municipal water facilities, the types of pathogens and indicators that water laboratories are generally interested in detecting and hardware requirements for biosensors that may potentially be used in a municipal water laboratory. 9:45 Biosensor Format and Technological Backbone for a “National Biodefense & Pandemie Immune System”
Peer F. Stähler, PhD, Vice President and Chief Scientific Officer, febit biotech GmbH, Germany
The unforeseeable nature of biodefense and pandemic threats suggest the development of a “National biodefense & pandemie immune system”. The technological backbone we propose provides: Fast and sensitive detection, ability to rapidly adapt and distribute tests, and decentralized test production to reduce vulnerability. A network of production sites feeds another network of analyzers. The components of this system are with units for flexible synthesis and instruments for analysis of high-speed microfluidic biochips equipped with microarrays of superb quality and 3'-oligos to enable polymerase based signaling. 10:15 Robust Sensing Platforms Based on Molecularly Tailored Xerogels
Frank V. Bright, PhD, Professor, Department of Chemistry, University at Buffalo, The State University of New York
Although biologically-based recognition elements (e.g., antibodies, aptamers, enzymes, etc.) are being used to detect biowarfare agents, there are compelling reasons to develop inexpensive, robust, and reusable alternatives for these expensive and unstable biorecognition elements. This presentation will describe new strategies that exploit sol-gel processing in concert with molecular templating to design highly stable, reagentless sensors without an expensive/labile biorecognition element. 10:45 Refreshment Break, Exhibit/Poster Viewing 11:15 Current Advances in HTP DNA Sequencing Technologies
Kenton L. Lohman, PhD, Senior Biotechnology Advisor, MRI Mid Atlantic Operations, Midwest Research Institute
HTP sequencing technologies have begun to reach commercial fruition and to accelerate the discovery of biodiversity across many species. Technological approaches vary from conventional amplification-based sample preparations to single molecule microchip platforms. The application of these new technologies to Biodefense is driving biomarker discovery for diagnostics, vaccines, and therapeutics to defend against natural or engineered biological threats. 11:45 Development of Assays and Qualified Reagents in Support of Biothreat Agent Detection
Kurt J. Langenbach, PhD, Assay Development Scientist, ATCC/Biodefense & Emerging Infections Research Resources Repository
Our program focuses on implementing increasingly rigorous levels of authentication and characterization to help establish standard reference biothreat organisms and nucleic acids. Assays and techniques that establish specimen identification, concentration, purity, functional activity and preservation/viability are evaluated. These may include biochemical profiling (using traditional and automated platforms), genetic profiling (such as sequencing, genome fingerprinting or PCR) and functional profiling (such as antibody reactivity/neutralization, or CPE). Our goal for these products and techniques is to extend the researcher’s ability to develop new technologies by enhancing the accuracy of pathogen identification. 12:15 Bringing Technology and Disciplines Together While Working With Wildlife as Biosensors
Nohra E. Mateus-Pinilla, PhD, DVM, Epidemiologist, Veterinary Pathobiology, Animal Sciences and Natural Resources and Environmental Sciences (Univ. Illinois, Urbana Champaign); Illinois Natural History Survey
Human activities have been associated with changes in biological diversity potentially increasing the risk of infectious diseases affecting people and livestock. Urbanization of landscapes augments interactions between humans and wildlife. In the meantime, urban wildlife is more likely to become in contact with both domestic animals and rural wildlife. The use of Wildlife as biosensors is a unique applied strategy with multiple applications. It allows us to monitor disease outbreaks and spread (i.e. WNV). It fosters active collaborations with multiple disciplines biologists, ecosystem managers, veterinarians (working with livestock or at State Animal Disease Laboratories), epidemiologists, GIS, modelers, public health departments, engineers and others. Native wildlife and natural ecosystems are long existing tools for biodetection of pathogens and other threats. 12:45 Luncheon Sponsored by The Knowledge Foundation Mass Spectroscopy, Host-Pathogen Interactions, Biomarker Discovery and Proteomic Detection 2:00 Detection and Quantification of Anthrax Lethal Factor by Mass Spectrometry
John R. Barr, PhD, Chief, Biological Mass Spectrometry Laboratory, Centers for Disease Control and Prevention*
Bacillus anthracis is the bacterium that causes anthrax. There are two binary toxins produced by B. anthracis which include a lethal factor (LF)-protective antigen (PA) complex known as lethal toxin and one which is a complex of edema factor (EF) and PA known as edema toxin. We have developed mass spectrometry based methods that can detect and quantify lethal factor in serum or plasma. The method can detect low levels of LF even in early stages of anthrax.
*In collaboration with: A.E.Boyer, C.P.Quinn, CDC 2:30 Host Responses to Biowarfare Agents: Exposure to Aerosol and Development of Immunity
Luis M. DaSilva, PhD, Microbiologist, Center for Aerobiological Sciences, USAMRIID - The United States Army Medical Research Institute for Infectious Diseases Abstract not available at time of printing.
Please visit this page frequently for the latest updates on the Program. 3:00 Biomarker Discovery using Bio-Rad’s SELDI-Based Technology
Amanda Bulman, PhD, Application Scientist, Bio-Rad Laboratories
Bio-Rad’s SELDI-based ProteinChip systems offer a single, unified platform for discovery and characterization of biomarkers of exposure, toxicity, and disease. SELDI facilitates rapid, high-throughput analysis of diverse sample types, including serum, urine, saliva, CSF, and cell and tissue lysates. Bio-Rad’s Biomarker Research Centers provide collaborative research services, allowing clients to access Bio-Rad’s internal expertise in study design and data analysis as well as novel technologies for enriching low abundance biomarkers. 3:20 One-Shot Kinetics Measurement with ProteOn XPR36
Sergei Bibikov, PhD, Application Scientist, Bio-Rad Laboratories
Bio-Rad presents a new type of SPR instrument with multiplexing capabilities for high precision measurements of kinetic and thermodynamic parameters of bio-molecular interactions in non-labeled samples. ProteOn XPR36 system offers a fast parallel processing of 36 bio-molecular interactions with its unique 6x6 system of microfluidic channels. Diversified surface chemistry helps to preserve high level of protein binding activity on the surface. Interspot reference adds to the precision of kinetic measurements. 3:40 Refreshment Break, Exhibit/Poster Viewing Multiplexed Detection Methods 4:10 Integrated Antibody Arrays for Identifying Infectious Diseases in Clinical Samples
Darrell P. Chandler, PhD, Vice President R&D, Akonni Biosystems Inc.*
Antibody arrays may significantly improve diagnostic confidence and/or detection limits of rapid immunoassays, while retaining time-to-result and ease-of-use metrics normally associated with the lateral flow test devices. In this presentation, we show multiplexed microarray detection of respiratory pathogens and toxins with Akonni TruArray™ substrates and portable imaging device, at levels equal to or greater than those achieved by comparable tests and equivalent reagents. Further integration of the multiplexed assay into Akonni’s microfluidic system will result in a low-complexity or CLIA-waived system for the doctor or technician to test for multiple respiratory pathogens or threat agents in one, rapid, simple to use, low cost test.
*In collaboration with: D.S.Schabacker, Argonne National Laboratory; C.J.Knickerbocker, C.E.Daitch, Akonni Biosystems Inc.; J.Walker, T.O’Brien, Tetracore Inc. 4:40 Biodetection using Bio-Plex™ Suspension Array System
Deyrick Dean, PhD, Application Scientist, Bio-Rad Laboratories
The Bio-Plex™ Suspension Array System, built upon xMAP technology (licensed from Luminex Corp.), permits the quantification of up to 100 different DNA, RNA, Peptide, or Protein targets from sample-limited biological fluids, or extracts. This presentation will discuss the key features of the Bio-Plex™ platform and its adoption by local, state, and federal public heath laboratories for detection of materials such as bioterrorism agents and foot and mouth disease (FMD). 5:00 Multiplex Lateral Flow Detection of Pathogens
Richard J. Obiso Jr., PhD, Director, Life Sciences, Luna Innovations, Inc.
To meet the urgent need for deployable diagnostics, Luna Innovations is developing a novel magnetic immunocapture lateral flow (MLF) device aimed at identifying the presence of biological threats and other microorganisms. The deployable assay will be employed at the point of care, have a 15 minute or less response time, will retain function in extreme environments, and have minimal logistics burden. Each assay operates in a multiplex capacity for the simultaneous detection of microorganisms. 5:30 Concluding Discussion, End of Day One Friday, June 15, 2007 8:00 Exhibit/Poster Viewing, Coffee and Pastries PCR and non-PCR Emerging Detection Technologies 8:45 Field Testing of a Fully Automated Real-Time PCR Device for the Detection of an RNA Virus
Martin A. Lee, PhD, Technical Manager, Enigma Diagnostics Ltd., United Kingdom*
This presentation describes the functioning and field trialling of equipment that enables nucleic acid-based testing for the detection of biological agents to be carried out by non-technical operators in non-laboratory settings. The equipment is portable, fully automated and can be operated from a battery, a vehicle or mains supply. Tests can be performed for either specific DNA sequences or RNA sequences which means that both bacterial and viral agents can be rapidly confirmed to “gold-standard” levels using a single, portable instrument in less than 45 minutes.
*In collaboration with: D.Squirrell. Enigma Diagnostics Ltd.; P.Wakeley, Veterinary Laboratory Agency 9:15 A Portable PCR System with Integrated Sampling and Detection
Brian Hicke, PhD, Chief Science Officer, Global Technologies (NZ) Ltd., New Zealand
We present a PCR system featuring very simple operation, rapid testing, portability, low cost, and integration of sample preparation with PCR. The system comprises a small and portable machine (10 kg), a laptop or handheld computer, and a plastic sampler. The disposable sampler collects material, is capped, and then placed into the analysis machine. With a single reagent addition, the system delivers a test within 35 minutes of sampling. 9:45 Reliable Sample Preparation with Isothermal Real-Time Nucleic Acids Amplification for Rapid Detection of Microorganisms
George Hong, PhD, R&D Manager, Technology and Pre-Development, Millipore Corporation
Here, we present a simple and innovative approach to concentrate organisms from liquid samples and detecting them by using the real-time transcription-mediated amplification technology (Real-Time TMA, Gen-Probe Incorporated). The combined technologies can potentially be applied to detecting a wide variety of microorganisms, including bacteria, viruses, mycoplasma, and fungi and has the potential sensitivity to detect as low as 1-5 CFU. Micro- and Nanoscale Based Technologies 10:15 Microtransponder-Based Biodetection System
Wlodek Mandecki, PhD, President, PharmaSeq, Inc.
PharmaSeq provides ultra-small electronic chips, microtransponders, and a fluidics-based, bench-top flow reader as tools to perform complex bioassays. The assay involves three phases, conjugation or synthesis of capture molecules (probes, antibodies) on microtransponders, the binding reaction with reporter molecules (targets) labeled with a fluorescent dye, and the readout of the fluorescence and the microtransponder’s ID by the flow reader. The system has been used in genotyping, protein and cell-based assays. 10:45 Refreshment Break, Exhibit/Poster Viewing 11:15 Nanoparticle Probes for Theranostic Applications
Arnold Kell, PhD, and Benoit Simard, PhD, Principal Research Officer, Steacie Institute for Molecular Sciences, National Research Council, Canada
Our group has synthesized a number of different nanoparticles over the past several years that exhibit unique optical (quantum dots, gold nanoparticles and dye-doped silica nanoparticles) and magnetic properties (superparamagnetic iron oxide nanoparticles). These properties lend themselves nicely to bioanalysis applications, where they can aid in the detection and identification of cells and biomarkers. This presentation will highlight our group’s efforts to develop surface-modified quantum dot, dye-doped silica and superparamagnetic iron oxide nanoparticle probes to aid in the detection and identification of a number of pathogenic species from biological samples. 11:45 Biosensing with Semiconductor Quantum Dot Conjugates
Igor L. Medintz, PhD, Center for Bio/Molecular Science and Engineering, Code 6900, Naval Research Laboratory
Luminescent semiconductor nanocrystals or quantum dots (QDs) are a prototypical nanoparticle with unique electronic and photophysical properties including broad absorption spectra coupled to size-tunable narrow symmetrical photoluminescent emission, high quantum yields and exceptional resistance to photobleaching and chemical degradation. These properties are increasingly being applied to numerous biosensing and probing configurations. We have already demonstrated QD-bioconjugate based sensors specific for nutrients, explosives and enzymatic activity. The unique potential of QDs suggests they will find use in many biodetection configurations. 12:15 Verigene® System: A Nanoparticle-Based Ultra-Sensitive Diagnostic Platform
Sudhakar Marla, PhD, Senior Scientist, Nanosphere, Inc.
Nanosphere’s Verigene® System, a nanoparticle-based diagnostic platform, allows high-fidelity, automated detection of ‘bio-markers’. DNA-modified gold nanoparticle probes enable multiplex ‘PCR-less’ detection of SNPs, infectious diseases, and bio-threat agents. The enzyme-free strategy is simple, robust, and free of variability associated with target amplification. The microarray platform also permits rapid detection of protein targets. An automated, ruggedized version of the Verigene System has been developed for the detection of bio-threat agents as well as bio-toxins. 12:45 Lunch on Your Own On-A-Chip Detection Technologies 2:00 Integration of Biology with Silicon Devices for Biological Detection: Opportunities and Future Prospects
Rashid Bashir, PhD, Professor, Birck Nanotechnology Center, Purdue University
Integration of biology with silicon devices and silicon-inspired fabrication promises to enable a wide range of applications in diagnostics, therapeutics, and tissue engineering. In this talk, we will present an overview of a range of projects in our group integrating micro-systems engineering & fabrication with biology, focused towards detection and characterization of biological entities using electrical or mechanical phenomenon at the micro and nano scale. Towards this end, we will present our work on developing silicon-based petri dishes-on-a-chip, nanomechanical cantilever sensors for detection of viruses, silicon based nano-pores for detection of DNA, and related projects. 2:30 Printed Reader on Chip
Max Sonnleitner, PhD, Chief Technology Officer, BioIdent Technologies, Inc.
Easy and cost-efficient integration of miniaturized components for optical readout still represents a major obstacle in developing compact mobile environmental monitoring systems. BioIdent solves this by printing organic photonics directly onto lab on a chip platforms. The novel concept allows for the first time to integrate complete illumination and detection capabilities onto microfluidic-based devices. Absorption and chemiluminescence readout data show the high sensitivity and dynamic range of the integrated photonics. 3:00 Lab-on-a-Chip-PCR - Continuous Flow PCR as Ultrafast Analytical Tool
Claudia Gärtner, PhD, CEO, microfluidic ChipShop GmbH, Germany
PCR is one of the dominating methods in life sciences. One major challenge is to speed up in time and to decrease sample volumes. A unique approach is the continuous-flow PCR on a microfluidic chip. Here, the conventional thermocycling is replaced by pumping the sample over stationary temperature zones. This allows not only to run a PCR in much less then 5 minutes, but also the option to integrate much more then just PCR on a chip. 3:30 Refreshment Break, Exhibit/Poster Viewing Fluorescence-Based Analytical Detection Systems 3:45 Standoff Spectral Detection of Bioaerosols by Laser-Induced Fluorescence
Sylvie Buteau, PhD, Defense Scientist; and
Jean-Robert Simard, PhD, Group Head, Optronic Surveillance Section, Defense Research and Development Canada (DRDC), Canada*
Defense Research and Development Canada (DRDC) has demonstrated a methodology based on Laser Induced Fluorescence (LIF) and intensified range-gated spectrometric detection to investigate standoff bioaerosol detection. The SINBAHD (for Standoff Integrated Bioaerosol Active Hyperspectral Detection) sensor, built for that purpose, has demonstrated impressive bioaerosol sensitivity and classification capabilities. In this presentation, the results associated with this innovative sensor to measure the spectral characteristics of various biological agent simulants, interferants and ambient bio-aerosols of natural and anthropogenic origins at multi-kilometre ranges will be presented and discussed.
*In collaboration with: P.Lahaie, G.R.P. Mathieu, B.Déry, H.Lavoie, J.McFee, and J.Ho 4:15 Native Fluorescence Spectroscopy On-A-Chip for Pathogen Detection
Peter Kiesel, PhD, Palo Alto Research Center*
Our compact, low-cost pathogen detection platform combines a fluidic channel with a chip-size, distributed spectrometer that is optimized to record fluorescence spectra from moving analytes. Large-volume, continuous fluorescence excitation is achieved by incorporating the fluidic channel into an anti-resonance waveguide that accommodates either laser diodes or LEDs. Simultaneously monitoring total intensity and spectrally-resolved emission yields accurate spectra for pathogen discrimination. System operation and pathogen detection will be demonstrated by video.
*In collaboration with: O.Schmidt, M.Bassler, N.Johnson 4:45 TIRF-EC Biosensors - Massively Parallel Dynamic DNA and Protein Microarrays for Accurate and Rapid Detection of Pathogens
Alexander N. Asanov, PhD, President, TIRF Technologies, Inc.
Total internal reflection fluorescence (TIRF) combined with electrochemistry and electric field control (TIRF-EC) is a platform technology, which is capable of detecting thousands of DNA/RNA and protein molecular markers in a matter of several seconds or a few minutes. The detection limit of TIRF-EC is at the level of single molecules. TIRF-EC biosensors are well suited for point-of-care and field applications, since they require no or minimum sample preparation stages. Cartridges of the TIRF-EC biosensors carry microarrays of assays for simultaneous detection of multiple DNA/RNA and proteins. In contrast to traditional DNA and protein arrays, TIRF-EC sensors monitor dynamics of association and dissociation, which allows for discriminating SNP in DNA/RNA targets and distinguishing between close homologs of proteins. In this presentation we report data on sensitivity, selectivity, and rate of responses for autonomous portable TIRF-EC biosensor. 5:15 Selected Poster Highlights and Concluding Discussion 5:30 Closing Remarks, End of Conference
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