Invited Speakers: Dr David Harris (Course Director)read Biochemistry at Oxford
University and conducted his post-doctoral work in Amsterdam
before returning to Oxford. After lecturing at Leeds University
he returned to Oxford once more and is currently a lecturer
Dr Mark Sansom studied as an undergraduate in Biochemistry
at Oxford University moving into Molecular Biophysics for
post-graduate work. In 1991 he returned from Nottingham
University - where he had been lecturing in Zoology - to
take up a lectureship at Oxford in Biochemistry and he is
currently a University reader in Biochemistry.
1. Protein Structure Basics
· Amino acids, peptides and proteins
· Local conformation – the Ramachandran plot
· Secondary structure – regularity within proteins
· Tertiary structure – folding the backbone
· Domains and modules
2. Protein Folds
· Databases of protein stucture – displaying folds
· All a folds e.g. 4 helix bundles. ‘Synthetic proteins’
· All b folds e.g retinol binding protein
· a/b folds – e.g.the TIM barrel and a+b folds – e.g. lysozyme
· multidomain proteins e.g. pyruvate kinase
3. Chemically modified proteins
· Chemical properties of amino acid side chains
· Directed protein modifiers as drugs – affinity labels and suicide substrates
· Covalent modification of proteins as a means of control
· Glycosylation – sites of protein glycosylation and the existence of glycoforms
4. Purifying proteins
· Releasing proteins from cells and membranes
· Protein surfaces – exploiting their features in purification
· Genetic engineering of proteins for ease of purification
· Chromatography in aqueous media – size exclusion, ion exchange, hydrophobic interactions
· Exploiting protein specificity – the design of affinity matrices
5. Determination of protein structures
· Study of secondary structure using CD and FTIR
· Protein nmr – principles and applications
· X-ray crystallography of proteins – principles and applications
· Electron microscopy and atomic force microscopy
· Computational methods – homology modelling and secondary structure prediction
6. Protein stability and folding
· Structures on the edge - stability of the native state of proteins
· Folding, unfolding and refolding – experimental methods in vitro
· The molten globule state
· Folding in the cell and the role of chaperonins
7. Enzymes and catalysis
· Theories of catalysis – can an enzyme be a ‘perfect’ catalyst?
· The active site concept – catalytic groups and ‘push-pull’ catalysis
· Activation entropy and strain – why enzymes are more than just chemical catalysts
· Roles of the enzyme surface in catalysis
8. Membrane proteins
· Classification of membrane proteins
· X-ray and electron diffraction methods – problems and achievements
· From sequence to topology – bioinformatics in developing and testing model structures
· Spectroscopic approaches
9. Analytical techniques
· Electrophoresis in gels and capillaries.
· Isoelectric focussing. 2D electrophoresis in gels
· Proteomics – visualising and identifying individual proteins
· Antibody blotting, mass spectrometry and sequencing in protein identification.
10. Antibodies and their use in analysis
· Structure of the antibody molecule
· Generation of antibody diversity. Monoclonal and polyclonal antibodies.
· Antibodies in identification of proteins in tissues – immunocytochemistry
· Quantitative use of antibodies –heterogeneous (e.g. ELISA) and homogeneous assays
· Sensitivity and ease of use in antibody-based assays.
Please register the following delegate for the course named below (for multiple
registrations please use copies of theis form):
Date of Course ______________________Registration Fee_£495.00_______________
Title (Dr/Mr/Ms etc)________________________________________________________
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Background: It is helpful for the course tutors to know the background of delegates; please could you provide the details listed below.
· Your main discipline (e.g. Biochemistry, Organic Chemistry, Molecular Biology, Pharmacology etc.)
· Your background (please circle) BSc/MSc/MD/PhD
PLEASE RETURN THIS COMPLETED FORM TO RACHEL BRISTOW:
University of Oxford, CPD Centre
67 St Giles
OX1 3LU or Fax the form to (+44) (0) 1865 288163
REGISTRATION TERMS AND CONDITIONS
Places are limited so early registration by application form is strongly recommended. This will assure a place on the conference.
Registration by Fax or Mail:
Upon request, a place on the conference can be reserved for individuals who require time to obtain authorisation and process the payment of fees. This can be done by faxing or mailing the completed and signed application form to Rachel Bristow (Fax No: +44 (0)1865 288163). Invoices will be issued immediately to those submitting application forms only.
Please note that enrolments are made on a strictly first-come first-served basis, and therefore bookings will only be confirmed after a completed application form has been received. Note also that the organisers reserve the right to refuse admission to any delegate who has not paid the registration in full before the conference starts. In the event of late bookings, payment in full will be required at the registration on the first day of the conference or it is our policy to request credit card details which will be held for three weeks before processing or until payment is received.
Those who register by mail and have not received confirmation within three weeks should call us to ensure that their application has not been mislaid. Any delegate who has not received a confirmation of registration five days prior to the scheduled date of a conference should ring the CPD Centre to confirm that the conference will convene as scheduled.
The full conference fee covers tuition, notes, lunches and daytime refreshments.
PAYMENT: Payment can be made in several ways: By cheque: payable to the OUDCE in pounds sterling. By credit card: using VISA, ACCESS or MASTERCARD. We are not able to accept any other forms of credit card. By Bank Transfer: please inform us and we will send the appropriate details.
This Programme is financially self-supporting, and it is necessary to establish a minimum enrolment in each conference. If the conference is cancelled by the organisers, registered delegates will be given as much notice as possible (normally at least 10 days prior to the conference start-date). However, our liability will be limited to the refund of the registration fees in full (without deductions). If a registration is cancelled by a delegate, full refunds of registration fees less £50 administration costs are payable for cancellations received in writing ten working days before the conference starts. After this date no fees are returnable. The organisers reserve the right to make alterations to the stated Programme and other arrangements as deemed necessary.
Registered delegates who cannot attend the conference may be substituted at any time. Please notify Rachel Bristow at the address given below as far in advance as possible, giving full details of the person who will attend in your place. A completed application form for the replacement should thereafter be faxed to the CPD Centre.
FURTHER INFORMATION: Queries about any aspects of the programme - including registration, accommodation, fees, etc. - should be referred to either:
Rachel Bristow, CPD Administrative Assistant, E-mail: email@example.com, Tel: +44 (0)1865 288162
Suzy Hodge, CPD Portfolio Manager E-mail: firstname.lastname@example.org, Tel: +44 (0)1865 288167
Fax: +44 (0)1865 288163
PLEASE RETURN FORM OVERLEAF TO RACHEL BRISTOW:
University of Oxford, CPD Centre
67 St Giles
or Fax the completed form to the number above
Deadline for Abstracts: not applicable
Email for Requests and Registration: email@example.com