Invited Speakers: Faculty: Dr. Michael J. Shamblott, Workshop Director, Johns Hopkins University School of Medicine; Dr. Suria Pancharatnam, JHU; Beth Bugg, JHU; Deborah Howard, JHU; Scott Durkin, ATCC.
Program:
This five-day laboratory-intensive workshop will provide intermediate and advanced instruction in cDNA cloning, detection, sequencing, and manipulation. Hands-on laboratory experience will be supplemented with lectures and discussions. Participants should be familiar with the techniques and concepts presented in the ATCC Recombinant DNA: Techniques and Applications workshop, such as molecular cloning, enzymes, vectors, E. coli host genotypes library construction, nucleic acid sequencing, restriction mapping, probes and PCR, and synthesis and cloning of cDNA.
This workshop will focus specifically on the detection and measurement of mRNA expression, cDNA analysis, and biosynthetic/transgenic techniques. The workshop will consist of hands-on exercises built around actual laboratory protocols. These exercises are supported by lectures pertaining to each of the techniques, as well as problem solving, and reagent preparation. We have chosen some of the most commonly employed techniques, such as Northern blotting, magnetic bead mRNA isolation, RTPCR, cDNA library construction, lipofection, and electroporation. These techniques can complement existing molecular biology research or can form the foundation for a new foray into this field.
For research material, we will use mouse embryonic stem (ES) cells, embryonic germ (EG) cells, and tissues. ES and EG cells are of particular interest, as their pluripotentcy allows for genetic manipulation of the mouse germ line, leading to gene knockOut and transgenic mice.
Students are encouraged to thoroughly read the pertinent lectures and laboratory exercises prior to their presentation. Lecture and exercise schedules are provided in the workshop manual. In addition to the manual, lectures, and exercises, we will work with the students to formulate questions and choose methods that are applicable to their own research goals.
Location: This workshop is co-sponsored by the Parenteral Drug Association (PDA) and will be held at the PDATraining and Research Institute in Catonsville, Maryland
Registration :
Limited to 24 participants
FEE: $1,395.00
3.5 CEUs
1-800-359-7370
workshops@atcc.org
Deadline for Abstracts: August 15, 1998
Email for Requests and Registration: workshops@atcc.org