Program:
Two hands-on, laboratory based courses on Recombinant DNA Technology will be offered at Indiana University this summer:
Recombinant DNA Technology, June 7-12.
Participants in this week-long course will learn the following techniques:
�DNA and cloning vector manipulation
�PCR technology
�Preparation of recombinant DNA
�Transformation of bacterial cells
�Selection and assay of cloned and amplified fragments of "foreign" DNA
�Southern blot analysis
�Preparation of nonradioactive DNA probes
�Transformation of yeast cells
�Analysis of signal transducing pathway using yeast two hybrid system
�Use of web sites in research and teaching
Application of Recombinant DNA Technology: RFLP and Fingerprinting Analysis, RAPD Analysis, and DNA Sequencing, June 14-19.
The following techniques will be emphasized:
�DNA sequencing
�RAPD analysis of genomic DNA
�Fingerprinting and RFLP analysis of genomic DNA
�Electroporation of bacterial cells
�Chemiluminescent detection of nucleic acids
�Application of computers to DNA sequencing data analysis
�Preparation of random fragment sequencing libraries and double-stranded DNA for sequencing
�Use of the BioNeb cell and biopolymer disruption system
�DNA sequencing using an automatic DNA sequencer
�Use of web sites for molecular biology
Registration :
Participants are expected to include
�college or high school educators who want to perform and teach these techniques
�industrial or other laboratory researchers considering the application of recombinant DNA technology
�others interested in the application of this technology in society
Cost is $1095 per course; discounted fee for both courses is $1800
Deadline for Abstracts: no abstracts
Email for Requests and Registration: jclay@indiana.edu