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Recombinant DNA: Techniques and Application

Indiana University , Indiana University - Bloomington, Indiana
June 7-12, 1998 and June 14-19, 1998

Program:
Two hands-on, laboratory based courses on Recombinant DNA Technology will be offered at Indiana University this summer:

Recombinant DNA Technology, June 7-12.

Participants in this week-long course will learn the following techniques:

•DNA and cloning vector manipulation
•PCR technology
•Preparation of recombinant DNA
•Transformation of bacterial cells
•Selection and assay of cloned and amplified fragments of "foreign" DNA
•Southern blot analysis
•Preparation of nonradioactive DNA probes
•Transformation of yeast cells
•Analysis of signal transducing pathway using yeast two hybrid system
•Use of web sites in research and teaching

Application of Recombinant DNA Technology: RFLP and Fingerprinting Analysis, RAPD Analysis, and DNA Sequencing, June 14-19.

The following techniques will be emphasized:

•DNA sequencing
•RAPD analysis of genomic DNA
•Fingerprinting and RFLP analysis of genomic DNA
•Electroporation of bacterial cells
•Chemiluminescent detection of nucleic acids
•Application of computers to DNA sequencing data analysis
•Preparation of random fragment sequencing libraries and double-stranded DNA for sequencing
•Use of the BioNeb cell and biopolymer disruption system
•DNA sequencing using an automatic DNA sequencer
•Use of web sites for molecular biology

Registration :
Participants are expected to include
•college or high school educators who want to perform and teach these techniques
•industrial or other laboratory researchers considering the application of recombinant DNA technology
•others interested in the application of this technology in society

Cost is $1095 per course; discounted fee for both courses is $1800





Deadline for Abstracts: no abstracts

Email for Requests and Registration: jclay@indiana.edu


Posted by: Jane Clay Host: clay.owen.indiana.edu date: February 02, 98 19:46:29
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