Invited Speakers: STEVE BROWN (Harwell), JAMES LUPSKI (Texas), STEVE OLIVER (Manchester), VAL SHEFFIELD (Iowa), ED SOUTHERN (Oxford), DANIELA TONIOLO (Pavia)
Program:
An intensive laboratory course to introduce participants to YAC, PAC, BAC and cosmid contig assembly, clone characterisation, gene hunting, expression and functional analysis
1. Generation of genomic contigs using YAC, PAC, BAC and cosmid-based techniques
2. FISH analysis of YAC and cosmid clones (demonstration only)
3. Retrofitting, insertional mutagenesis and fragmentation of YACs
4. Gene hunting
5. Expression profiling
6. Transfer of large constructs to mammalian cells
7. Subcellular localisation of proteins
8. Bioinformatics in genome analysis
Informal tutorials and evening lectures (by invited speakers) will supplement the laboratory sessions.
Course Instructors:
DUNCAN CAMPBELL (Oxford), NIGEL CARTER (Hinxton), MIKE DIXON (Manchester), TOM FREEMAN (Hinxton), DAVID MARKIE (Dunedin), JOS MEJĦA (Oxford), TONY MONACO (Oxford), IOANNIS RAGOUSSIS (London), GARY WILLIAMS (Hinxton), CAROL WISE (Texas)
Registration :
Not applicable
Deadline for Abstracts: Deadline for receipt of applications is April 3rd, 1998; please see http://www.umds.ac.uk/wlmg for information on how to apply
Email for Requests and Registration: wss@umds.ac.uk