Invited Speakers: Janet D. Rowley, David H. Ledbetter , David C. Ward , Jeffrey M. Trent
Program:
This course will focus on advanced molecular cytogenetic methodologies for the visualization of chromosome aberrations. Emphasis will be placed on applications of spectral karyotyping (SKY) for the refinement of clinical and tumorcytogenetic diagnostics. In addition, we will cover comparative genomic hybridization (CGH), and novel, sensitive methods for visualization of FISH-experiments (tyramides). Methodologies, technologies, and scientific expertise for the microscopic visualization of chromosomal alterations will be provided. The course is designed for cytogeneticists who use chromosome analytical methods in diagnostics and basic research and for the molecular biologists who intend to employ advanced molecular cytogenetic methods in mammalian chromosome analysis. Among the methods presented will be the preparation of tagged DNA probes, fluorescence in situ hybridization and multicolor detection methods, quantitative digital image analysis, spectral imaging, and data interpretation. Students are encouraged to bring their own samples of human or mouse origin for SKY and CGH analysis. State of the art microscopic and imaging equipment will be available to gain hands on experience.
Registration :
Information on how to apply at http://www.cshl.org/meetings
Deadline for Abstracts: January 15, 1998
Email for Requests and Registration: meetings@cshl.org