EARLY DEVELOPMENT OF XENOPUS LAEVIS

Cold Spring Harbor Laboratory , Cold Spring Harbor Laboratory
April 8 - 17, 1997

Invited Speakers: 1997 Xenopus Course Lecturers include:
Ken Cho "Molecular mechanisms of activin and BMP signaling during Xenopus embryogenesis"
Jan Christian "The role of proprotein convertases in vertebrate embryonic patterning."
Richard Harland "Expression cloning of developmental genes"
Janet Heasman "Adhesion molecules and antisense oligonucleotide inhibition of function".
Ray Keller "Xenopus gastrulation"
Dan Kessler "Regulation of Vertebrate Cell Fate by Maternal Inducers".
Paul Krieg "Homeobox genes in cardiovascular development"
Sally Moody "Cell-cell interactions and lineage influence expression of specific neuronal phenotypes"
Jonathan Slack "The role of fibroblast growth factors in Xenopus development"

Program:
This course will provide extensive laboratory exposure to the biology, manipulation and use of embryos from the frog,Xenopus Laevis. The course is suited both for investigators who have had no experience with Xenopus, as well as those who worked with Xenopus and wish to learn new techniques. All students should have a current training in molecular biology and some knowledge of developmental biology. The course consists of intensive laboratory sessions, supplemented by daily lectures and demonstrations from experts in both experimental and molecular embryology.

Areas to be covered include :
(i) care of adults and embryo isolation
(ii) stages of embryonic development and anatomy
(iii) whole mount in situ hybridization and immunocytochemistry
(iv) microinjection of eggs and oocytes, including DNA constructs, mRNA and antisense oligonucleotides
(v) micromanipulation of embryos, including explant and transplantation assays (vi) use of cell cycle extracts, including preparation of transgenic embryos.

Registration :
Information on how to apply for CSHL courses is available at http://www.cshl.org/meetings

Deadline for Abstracts: Application Deadline: January 15 1997

Email for Requests and Registration: meetings@cshl.org