PROTEIN PURIFICATION & CHARACTERIZATION
April 8 - 21, 1997
Invited Speakers: Last year's program of guest and instructor lectures:
Richard Burgess, Prof. of Oncology, Univ. of Wisconsin,
Madison, WI
Overview of Protein Purification, Immunoaffinity
Purification
Seth Darst, Rockefeller University, NY
Preparation and Analysis of Proteins for Structural Studies:
Applications to E. coli RNA Polymerase
Nick Pace, Prof. of Chemistry, Texas A&M, College
Station, TX
Protein Folding and Stability
Sue-Hwa Lin, Molecular Pathology, M.D. Anderson
Cancer Center, Houston, TX
Purification of Membrane Proteins
Jim Kadonaga, Dept. of Biology, UCSD, LaJolla CA
Transcription, Chromatin, and Purification of Nuclear
Proteins
Al Courey, Dept. of Chemistry and Biochemistry,
UCLA
Transcriptional Regulation of Dorsal/Ventral Pattern
Formation in Drosophila
Guido Guidotti, Molec. and Cell Biol., Harvard Univ.
Cambridge, MA
Does Insulin Receptor Function as a Dimer?
John Edsall, Molec. and Cell Biol., Harvard Univ.
Cambridge, MA
Reflections on 60 Years of Protein Purification
Dan Marshak, V.P. for R&D, Osiris Therapeutics,
Baltimore, MD
Phosphorylation and Regulation of Cell Growth
James Rothman, Memorial Sloan-Kettering Cancer
Center, NY
Protein Machinery of Vesicular Transport
Bruce Stillman, Director, Cold Spring Harbor
Laboratory
Biochemical Approach to Understanding the Replication of
Eukaryotic Cell Genome
Sheenah Mische, Director, Protein-DNA Technology
Center, Rockefeller University, NY
Microanalytical Protein Preparation and Characterization
Program:
This course is intended for scientists who are not familiar with techniques of protein isolation and characterization. It is a rigorous program that includes laboratory work all day and a lecture with discussion session every evening. Each student will become familiar with each of the major techniques in protein purification by actually performing four separate isolations including:
(i) a regulatory protein from muscle tissue
(ii) a sequence-specific, DNA binding protein
(iii) a recombinant protein overexpressed in E. coli
(iv) a membrane-bound receptor
A variety of bulk fractionation, electrophoretic, and chromatographic techniques will be employed including: precipitation by salts, pH, and ionic polymers; ion exchange, gel filtration, hydrophobic interaction, and reverse phase chromatography; lectin affinity, ligand affinity, oligonucleotide affinity, and immunoaffinity chromatography; polyacrylamide gel electrophoresis, and electroblotting; and high performance liquid chromatography. Procedures will be presented for solubilizing proteins from inclusion bodies and refolding them into active monomeric forms. Methods of protein characterization will be utilized including immunological and biochemical assays, peptide mapping, amino acid analysis, protein sequencing, and mass spectrometry. Emphasis will be placed on strategies of protein purification and characterization rather than on automated instrumental analysis. Guest lecturers will discuss protein structure, modification of proteins, methodologies for protein purification and characterization, and applications of protein biochemistry to cell and molecular biology.
Registration :
Information on how to apply for CSHL courses is available at http://www.cshl.org/meetings
Deadline for Abstracts: January 15, 1997
Email for Requests and Registration: meetings@cshl.org