ADVANCED GENOME SEQUENCE & ANALYSIS
March 14 - 27, 1997
Invited Speakers: Last year's program of lectures:
James Watson, Cold Spring Harbor Laboratory.
How the Human Genome Project got started
Douglas Smith, Collaborative Research, Inc.
Large scale multiplex sequencing
William Studier, Brookhaven National Laboratory.
DNA sequencing by primer walking with hexamer strings
Randall Smith, Baylor College of Medicine.
Sequence alignment and database searching: A practical guide for Molecular Biologists
Thomas Marr, Cold Spring Harbor Laboratory.
From pedigrees to sequences: Dissection of complex genetic diseases by computer
Bruce Roe, University of Oklahoma.
Human genomic sequencing: Strategies, results, analyses, and lessons learned from approximately one megabase
Thomas Caskey, Merck Research Laboratories.
Triplet repeat diseases
Robert Weiss, University of Utah.
Automated multiplex sequencing: Technology development and applications
Andreas Dusterhoft, Qiagen GmbH. Germany.
New developments in template purification for genome sequencing projects
Program:
Recent advances in the automation of DNA sequencing have opened new possibilities for the analysis of complex genomes at the DNA sequence level. This two week course will provide intensive training in this rapidly evolving field. The course will emphasize techniques and strategies for using automated sequences to sequence large, contiguous genomic regions. Students will carry out all of the steps in the sequencing process from preparing cosmid DNA to computer analysis of the finished sequence. Topics will include subclone library generation, large-scale template purification, sequencing reactions, gel analysis on automated sequencers, sequence assembly, gap filling and conflict resolution, Students will work in groups to sequence a large region of DNA. In the last year's course a 45kb cosmid was sequenced (GenBank) accession #U23729). Through this process they will be trained in crucial project and data management techniques. A series of lecturers will discuss their applications of these techniques as well as alternate strategies for high speed automated DNA sequencing.
Registration :
Information on how to apply for CSHL courses is available at http://www.cshl.org/meetings
Deadline for Abstracts: Application Deadline: January 15 1997
Email for Requests and Registration: meetings@cshl.org