POLYMERASE CHAIN REACTION METHODOLOGY
February 3-7, 1997
Invited Speakers:
Program:
DAY 1
Lectures
PCR I: The Polymerase Chain Reaction (PCR and DNA Amplification)
Sample Preparation for PCR - Nucleic Acid Isolation Methods
Laboratory
Isolation of RNA from Cell Samples for PCR Amplification
Symmetric DNA Amplification
DAY 2
Lectures
PCR II: Optimization of PCR Components and Product Analysis
PCR III: Related Techniques and Uses of PCR
Laboratory
Symmetric PCR Product Analysis
DNA/RNA Sample Analysis
Purification of poly(A+) RNA by Magnetic Bead Technology
Amplification of RNA by PCR (RT-PCR)
DAY 3
Lectures
PCR IV: Practical Do's and Don'ts for the PCR Enthusiast
PCR V: A Mainstream Research Tool for the Molecular Biologist
Laboratory
RNA PCR Product Analysis
Asymmetric PCR
Analysis of Asymmetric PCR Products
PCR Mutagenesis
Southern Blot Analysis of PCR Products (I)
DAY 4
Lectures
PCR VI: Practical Applications
Non-Isotopic Methods for Probe Labeling and Detection
Bringing it all Together
Laboratory
Analysis of Mutagenesis Products
Cloning PCR Products (Ligation/Transformation/Plating)
Southern Blot Analysis of PCR Products (II)
Non-Isotopic Nucleic Acid Probe Synthesis
Discussion of Specific Research Interests
DAY 5
Laboratory
Identification and Analysis of Cloned PCR Products
Post-Hybridization Washes of all PCR Product Southern Blots
Hybridization Detection of all Blots by Chemiluminescence
Digital Image Analysis for the Molecular Biologist
Final Discussion
Tuition: Tuition for each workshop is $1150.00 US. and includes all notes, protocols, lab supplies and lunch each day of the workshop. Please note that travel lodging and other meals are the responsibility of each participant.
Registration :
Scientists who wish to enroll in this workshop shall procure a bank draft drawn on US funds only made payable to "Exon-Intron". Registration forms may be obtained by accessing our web site http://www.DNAtech.com or by FAX (+410-730-3983). Completed registration forms and tuition payments should be mailed to:
Exon-Intron
9151 Rumsey Road
Suite 130
Columbia, MD 21045 USA
Registrations will be handled on a first come-first served basis, and advance payment of tuition is required. A receipt and confirmation of registration will be mailed when registration form and tuition are received.
Accommodations: A block of rooms has been reserved at the Hotel Mozart in Innsbruck. Workshop participants are given a university rate, as long as they inform the hotel that they will be attending the workshop(s) at the time reservations are made. This hotel is 5 minutes walking distance from the University location where the workshops will be conducted. The hotel strongly recommends early reservations.
Hotel Mozart
Mullerstrasse 15
A-6010 Innsbruck
Austria
Tel: +43-512-59538
FAX: +43-512-595-386
Dormitory-style student housing is also available: Contact:
INNSBRUCK INFORMATION
Burggraben 3
A-6020 Innsbruck
Austria
Tel: +43-512-5356.0
FAX: +43-512-535643
Ask for Mrs. Strauss
For information pertaining to other aspects of your visit to Austria:
http://harvest.austria.eu.net/server-in-AT/index.html
http://info.uibk.ac.at/tyrol/index-de.html
http://info.uibk.ac.at (English)
http://info.uibk.ac.at/c115/index.html (German)
http://www.ifs.univie.ac.at/austria/ssk.html
Questions:
Please do not hesitate to contact us by telephone, FAX or e-mail
Workshop Coordinator
Telephone: +410-730-3984
FAX: +410-730-3983
e-mail: workshop@DNAtech.com
Web Site: http://www.DNAtech.com
As always, be sure to visit our web site for information about other workshops or to add yourself to our mailing list.
Deadline for Abstracts: n/a
Email for Requests and Registration: workshop@DNAtech.com