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POLYMERASE CHAIN REACTION METHODOLOGY

, University of Innsbruck, Institute of General & Experimental Pathology
February 3-7, 1997

Invited Speakers:

Program:

DAY 1

Lectures


PCR I: The Polymerase Chain Reaction (PCR and DNA Amplification)
Sample Preparation for PCR - Nucleic Acid Isolation Methods

Laboratory

Isolation of RNA from Cell Samples for PCR Amplification
Symmetric DNA Amplification


DAY 2

Lectures


PCR II: Optimization of PCR Components and Product Analysis
PCR III: Related Techniques and Uses of PCR

Laboratory

Symmetric PCR Product Analysis
DNA/RNA Sample Analysis
Purification of poly(A+) RNA by Magnetic Bead Technology
Amplification of RNA by PCR (RT-PCR)


DAY 3

Lectures


PCR IV: Practical Do's and Don'ts for the PCR Enthusiast
PCR V: A Mainstream Research Tool for the Molecular Biologist

Laboratory

RNA PCR Product Analysis
Asymmetric PCR
Analysis of Asymmetric PCR Products
PCR Mutagenesis
Southern Blot Analysis of PCR Products (I)


DAY 4

Lectures


PCR VI: Practical Applications
Non-Isotopic Methods for Probe Labeling and Detection
Bringing it all Together

Laboratory

Analysis of Mutagenesis Products
Cloning PCR Products (Ligation/Transformation/Plating)
Southern Blot Analysis of PCR Products (II)
Non-Isotopic Nucleic Acid Probe Synthesis
Discussion of Specific Research Interests

DAY 5

Laboratory


Identification and Analysis of Cloned PCR Products
Post-Hybridization Washes of all PCR Product Southern Blots
Hybridization Detection of all Blots by Chemiluminescence
Digital Image Analysis for the Molecular Biologist
Final Discussion


Tuition: Tuition for each workshop is $1150.00 US. and includes all notes, protocols, lab supplies and lunch each day of the workshop. Please note that travel lodging and other meals are the responsibility of each participant.

Registration :

Scientists who wish to enroll in this workshop shall procure a bank draft drawn on US funds only made payable to "Exon-Intron". Registration forms may be obtained by accessing our web site http://www.DNAtech.com or by FAX (+410-730-3983). Completed registration forms and tuition payments should be mailed to:

Exon-Intron
9151 Rumsey Road
Suite 130
Columbia, MD 21045 USA

Registrations will be handled on a first come-first served basis, and advance payment of tuition is required. A receipt and confirmation of registration will be mailed when registration form and tuition are received.

Accommodations: A block of rooms has been reserved at the Hotel Mozart in Innsbruck. Workshop participants are given a university rate, as long as they inform the hotel that they will be attending the workshop(s) at the time reservations are made. This hotel is 5 minutes walking distance from the University location where the workshops will be conducted. The hotel strongly recommends early reservations.

Hotel Mozart
Mullerstrasse 15
A-6010 Innsbruck
Austria
Tel: +43-512-59538
FAX: +43-512-595-386

Dormitory-style student housing is also available: Contact:

INNSBRUCK INFORMATION
Burggraben 3
A-6020 Innsbruck
Austria
Tel: +43-512-5356.0
FAX: +43-512-535643
Ask for Mrs. Strauss


For information pertaining to other aspects of your visit to Austria:

http://harvest.austria.eu.net/server-in-AT/index.html
http://info.uibk.ac.at/tyrol/index-de.html
http://info.uibk.ac.at (English)
http://info.uibk.ac.at/c115/index.html (German)
http://www.ifs.univie.ac.at/austria/ssk.html

Questions:

Please do not hesitate to contact us by telephone, FAX or e-mail
Workshop Coordinator
Telephone: +410-730-3984
FAX: +410-730-3983
e-mail: workshop@DNAtech.com
Web Site: http://www.DNAtech.com

As always, be sure to visit our web site for information about other workshops or to add yourself to our mailing list.

Deadline for Abstracts: n/a

Email for Requests and Registration: workshop@DNAtech.com


Posted by: Exon-Intron Host: klipha2.ukbf.fu-berlin.de date: December 02, 96 23:39:28
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