PCR APPLICATIONS / CYCLE DNA SEQUENCING

American Type Culture Collection , Rockville, Maryland, USA
April 9-12, 1996

Invited Speakers: Faculty: William Nierman, Ph.D., Director, ATCC Program in
Molecular Biology and Virology; Donna Maglott, Ph.D., Research Scientist; Yvonne
Reid, Ph.D., Collection Scientist; Francis Molina, Ph.D., Research Scientist; Tamara
Feldblyum, M.S., Collection Scientist.

Program:
This four-day, laboratory-intensive, workshop covers a broad range of applications of the polymerase chain reaction (PCR) for addressing biological problems. Included will be a DNA sequencing experiment using a thermostable DNA polymerase and a temperature cycling protocol (cycle sequencing). This workshop will provide introductory and intermediate level instruction in the application, procedures and trouble-shooting for this extremely useful and versatile technology.

A basic knowledge of nucleic acid laboratory procedures is helpful, but not necessary.
Approximately 75% of the course is devoted to laboratory instruction while 25% is
lecture-oriented. An extensive manual with protocols and procedures is provided for
each participant.

Lecture topics will include PCR Reaction Basics, Optimizing and Troubleshooting the
PCR Reaction, DNA Sequencing, PCR Analysis of Ribosomal DNA to Determine
Taxonomic Relatedness, PCR Fingerprinting, PCR in the Diagnosis of Infectious and
Genetic Disease, and PCR and Recombinant DNA Methodology. Time will be
structured for presentation and discussion of workshop participants' PCR
applications/problems.

The workshop is intended to be a laboratory-intensive course. Laboratory exercises will
include:
1) PCR Diagnosis of Sickle Cell Disease;
2) Fingerprint Analysis of the DNA of Human Individuals;
3) Relatedness of Organisms by Analysis of Amplified Ribosomal DNA;
4) Amplification from mRNA;
5) Optimization of PCR Reactions;
6) Labeling of Hybridization Probes by PCR;
7) Cycle DNA Sequencing; and
8) Fingerprinting of Microorganisms by RAPD and Inter-repeat PCR. Results will be analyzed and discussed.

* The polymerase chain reaction (PCR) process is covered by patents owned by
Hoffmann-La Roche. Use of the PCR process requires a license.

Limited to 25 participants
FEE: $1,095.00
2.8 CEUs

Registration :
On line registration is available.

Deadline for Abstracts: Registrations are handled on a first come, first served basis. Early registration discounts (60 days), multiple attendee discounts (2 or more), and University discounts are available.

Email for Requests and Registration: workshops@atcc.org