IMAGING STRUCTURE & FUNCTION IN THE NERVOUS SYSTEM
July 24 - August 13, 1996
Invited Speakers: Lecturers in the 1995 course included W. Betz, J. Connor, S. Fraser, T. Inoue, R. Lewis, L. Cohen, G. Ellis-Davies, J. Heuser, L. Katz, M. Minsky, J. Swedlow, J. White, R. Tsien, and W. Webb.
Program:
Advances in light microscopy, digital image processing, and the development of a variety of powerful fluorescent probes present expanding opportunities for visualizing and measuring the structure and function of neurons, synapses, and networks in the brain. This intensive laboratory and lecture course will provide participants with the theoretical and practical tools to utilize these emerging technologies. The primary emphasis of the course will be on light microscopy, including fluorescence, differential interference and phase contrast, confocal scanning, and 2-photon scanning, as well as the use of different types of electronic cameras and the application of digital processing to enhance and analyze microscope images. Students will learn the principles of light microscopy, the use of calcium-sensitive probes (e.g., Fura-2), voltage-sensitive dyes, photo-activated ("caged") compounds, exocytosis tracers, whole-cell patch-clamp methods, single-cell microinjection of fluorescent indicators, and other methods to explore neuronal function. We will use a variety of neural systems, including living animals, brain slices (e.g., hippocampus, cerebellum, and neocortex), and cultured cells. Applicants should have a strong background in the neurosciences or in cell biology.
Registration :
Information on how to register online is available.
Deadline for Abstracts: Application Deadline is March 15, 1996
Email for Requests and Registration: meetings@cshl.org