MOLECULAR CLONING AND EXPRESSION OF NEURAL GENES
July 1 - 21, 1996
Invited Speakers: Last year’s lecturers were: S. Burdon, A. Craig, R. Darnell, S. Fields, B. Hoffman, G. Limke, G. Mandel, P. Mombaerts, A. Quinn, P. Worley.
Program:
This intensive laboratory and lecture course is intended to provide neuroscientists at all levels with an introduction to modern molecular neurobiology. The course consists of daily laboratory exercises, discussions on the practice of molecular biology, and a series of evening research seminars by invited speakers. This lecture series will emphasize the ways in which molecular techniques studied in the laboratory have been successfully applied to the study of neural genes.
The laboratory portion of the course will include a module on gene cloning and a module on the analysis of neural gene expression. Gene cloning techniques will include isolation and characterization of poly (A+) RNA; synthesis of cDNA libraries; standard screening and expression screening of cDNA libraries; analysis of cDNA clones, including phagemid rescue, restriction analysis, Southern blotting, ligations, transformation, electroporation and subcloning; PCR analysis including oligonucleotide design, synthesis, and purification, and PCR-based subtraction cloning. Gene expression studies will include Northern blot analysis, RT-PCR analysis, in vitro transcription for RNAase protection, in situ hybridization, mammalian cell transfection, and an exploration of techniques used for in vivo analysis of neuronal gene expression including the use of viral vectors, expression with a "gene gun", and mammalian gene targeting.
Registration :
Information on how to register online is available.
Deadline for Abstracts: Application Deadline is March 15, 1996
Email for Requests and Registration: meetings@cshl.org