MOLECULAR APPROACHES TO ION CHANNEL BIOLOGY
June 7 - 27, 1996
Program:
This intensive laboratory/lecture course will introduce students to the multidisciplinary use of molecular biological, biochemical, immunological, and electrophysiological approaches to the study of ion channels. The laboratory will focus on the cellular regulation of channel expression and function. Hands-on exercises will include characterization of regulatory elements that control transcription of channel genes, identification of ion channel isoform transcripts and proteins, visualization of channel distributions, and the biophysical analysis of channel isoform function in excitable cells and tissues and in exogenous expression systems. Specific techniques employed will include PCR, western blot, transient transfection and expression, immunocytochemistry, and patch clamp analysis of ionic currents. Lectures will cover the techniques employed while providing broad exposure to current issues surrounding ion channel mechanisms and cellular expression. This course is intended for advanced students with specific plans to apply the techniques taught to a defined problem, and students are encouraged to bring their preparation to the course for preliminary studies.
Registration :
Information on how to register online is available.
Deadline for Abstracts: Application Deadline is March 15, 1996
Email for Requests and Registration: meetings@cshl.org