PROTEIN PURIFICATION & CHARACTERIZATION
April 10 - 23, 1996
Invited Speakers: Guest lecturers will discuss protein structure, modification of proteins, methodologies for protein purification and characterization, and applications of protein biochemistry to cell and molecular biology. Guest lecturers have included: R. Aebersold, L. Gierasch, G. Hart, A. Kornberg, N. Pace, Y. Paterson, G. Rose, J. Rothman, B. Stillman, and N. Tonks.
Program:
This course is intended for scientists who are not familiar with techniques of protein isolation and characterization. It is a rigorous program that includes laboratory work all day and a lecture with discussion session every evening. Each student will become familiar with each of the major techniques in protein purification by actually performing four separate isolations including: (i) a regulatory protein from muscle tissue: (ii) a sequence-specific, DNA binding protein: (iii) a recombinant protein overexpresed in E. coli; and (iv) a membrane-bound receptor. A variety of bulk fractionation, electrophoretic, and chromatographic techniques will be employed including: precipitation by salts, pH, and ionic polymers; ion exchange, gel filtration, hydrophobic interaction, and reverse phase chromatography; lectin affinity, oligonucleotide affinity, and immunoaffinity chromatography; polyacrylamide gel electrophoresis, and electroblotting; and high performance liquid chromatography. Procedures will be presented for solubilizing proteins from inclusion bodies and refolding them into active monomeric forms. Methods of protein characterization will be utilized including immunological and biochemical assays, peptide mapping, amino acid analysis, protein sequencing, and mass spectrometry. Emphasis will be placed on strategies of protein purification and characterization rather than on automated instrumental analysis.
Registration :
Information on how to register is available online.
Deadline for Abstracts: Application Deadline is January 15, 1996
Email for Requests and Registration: meetings@cshl.org