ADVANCED GENOME SEQUENCE ANALYSIS
March 20 - April 2, 1996
Invited Speakers: Last year's speakers included: C.T. Caskey, A. Dusterhoft, T. Marr, D. Smith, R. Smith, F. W. Studier, B. Roe, R. Weiss, and J.D. Watson.
Program:
Recent advances in the automation of DNA sequencing have opened new possibilities for the analysis of complex genomes at the DNA sequence level. This two week course will provide intensive training in this rapidly evolving field. The course will emphasize techniques and strategies for using automated sequences to sequence large, contiguous genomic regions. Students will carry out all of the steps in the sequencing process from preparing cosmid DNA to computer analysis of the finished sequence. Topics will include subclone library generation, large-scale template purification, sequencing reactions, gel analysis on automated sequencers, sequence assembly, gap filling and conflict resolution, Students will work in groups to sequence a large region of DNA. In the last year's course a 45kb cosmid was sequenced (GenBank) accession #U23729). Through this process they will be trained in crucial project and data management techniques. A series of lecturers will discuss their applications of these techniques as well as alternate strategies for high speed automated DNA sequencing.
Registration :
Information on how to register is available online.
Deadline for Abstracts: Application Deadline is January 15, 1996
Email for Requests and Registration: meetings@cshl.org