Invited Speakers: Dr. David Katz, Dr. Steven Schrader, Dr. Susan Rothmann
Program:
Wednesday Evening
5:00 PM Orientation to Lab
6:00 PM Reception and Buffet Dinner
Thursday
8:30 AM Sperm Count Quality Control
Exercise 1 Establish baselines
Count, using your method
Exercise 2 Influence of Sample Size
Count sets of 10 squares
Exercise 3 Influence of Chamber
10:30 AM Data Analysis and Discussion
Statistical Process Control
12:00 noon Lunch on your own
1:00 PM Motility Quality Control
Exercise 1 Establish Baselines
Count using your method
Exercise 2 Influence of Sample Size
Count sets of 10 squares
2:00 PM Data Analysis and Discussion
Exercise 3 Live Counts vs Dead Counts
Count Appropriate # of Squares
Exercise 4 Influence of Chamber
Makler/Microcell/Cell Vu
3:30 PM Data Analysis and Discussion
Friday
8:30 AM Introduction to CASA - Dr. Daid Katz
I. Machine Vision and Semen Analysis
What do we want it to do?
A. Objective Measure
B. Repeatable
C. Permanent Record
II. CASA--How does it work?
A. Define terms
B. Basics of imaging &
computer image analysis
9:00 AM State of the Art - Dr. David Katz
What We Know So Far About
CASA Pluses and Pitfalls
1. Diagnostic usefulness
2. Technological challenges
3. # of sperm per field
4. Sperm counts
5. Common errors in analysis
9:30 AM CASA Exercises
Each Group will have 2 hours to complete
a series of identical experiments designed to
address the following:
1. How each instrument overcomes
technological challenges
2. Sample preparation methods
3. Basic operation for count and motility
4. QC and calibration
5. Kinematics
11:30 AM Lunch on you own
12:30 PM CASA Exercises
2:30 PM Break
3:00 PM CASA Exercises
Saturday
8:30 AM Discussion of CASA Data
10:30 AM Adjourn
Registration :
Course Fee: $850 Pre-registration before December 1, 1995; Registration $1000 after December 1, 1995
Send to: ASA, P.O. Box 15171, Lenexa, KS 66285-5171 Phone:(913) 541-9077 FAX:(913)541-0156
Deadline for Abstracts: none
Email for Requests and Registration: sms@niobbs1.em.cdc.gov