Invited Speakers: Faculty: William Nierman, Ph.D. (Workshop Director), Director, ATCC Molecular Biology Dept.; Donna Maglott, Ph.D., Associate Staff Scientist; Yvonne Reid, Ph.D., Associate Staff Scientist; Francis Molina, Ph.D., Associate Staff Scientist; Tamara Feldblyum, M.S., Assistant Collection Manager; Scott Durkin, B.S., Laboratory Technician.
Program:
This four-day laboratory-intensive course covers a broad range of applications of the
polymerase chain reaction (PCR) for addressing biological problems. Included will be a
DNA sequencing experiment using a thermostable DNA polymerase and a temperature
cycling protocol. The workshop will provide introductory and intermediate level
instruction in the application, procedures and trouble-shooting for this extremely useful
and versatile technology.
A basic knowledeg of nucleic acid laboratory procedures is helpful, but not necessary.
Approximately 75% of the course is devoted to laboratory instruction while 25% is
lecture-oriented. An extensive manual with protocols and procedures is provided for
each participant.
Lecture topics will include PCR reaction basics, optimizing and troubleshooting the
PCR reaction, DNA sequencing, PCR analysis of ribosomal DNA to determine
taxonomic relatedness, PCR fingerprinting, PCR in the diagnosis of infectious and
genetic disease, and PCR and recombinant DNA methodology. Time will be structured
for presentation and discussion of workshop participants PCR applications and
trouble-shooting.
Registration :
FEE: $1,050.00 Limited to 25 Participants
Deadline for Abstracts: N/A
Email for Requests and Registration: ahudak@atcc.org