A) Aquatic Plant Agronomy & Environmental Remediation Using Closed-Loop Animal-Fed Fertilized Pond Bed Culturement. Water hyacinth, duckweed and seagrass. These plants are to be assessed as feeds with livestock as to: a) acceptability/palatability, b) nutritive value (NV) specifically N balance and c) postharvest processing as hay or silage and solid-substrate fermentation (SSF) biomass, with the potential for high-quality (HQ) feedstock as feed and for biofuel manufacturing breakthroughs in terms of economics. All classes of livestock including pigs, chickens, goats, sheep, cattle and dairy may be fed on these aquatic-based forages.
B) Feeding Hi-Sugar Forages Bred Using Marker Assisted Selection (MAS) and Other non-GMO Approaches Including Protoplast Fusion & Protein Protection by Heat Damage and Use of Tannins for Browse Trees and Shrubs. These are all to be further verified with the aid of molecular probes consisting of protein nucleic (PNA)-B12 carriered gene silencing techniques as to the mechanisms at the molecular level on rumen microbial mitogenesis as further theorized by D. A. Flores (principal investigator) based on water-soluble carbohydrates and pre-formed amino acids effects on cell-cycle regulatory mechanism(s).
C) Dualized-Purposed Forages with Ureolytic Ensilage with Yeast. Dual-purpose (food and feed residuals further nutrified) fodders can be nitrogen (N) supplemented via ammoniation and delignification using direct-applied protein nucleic acid (PNA)-B12 carriered gene silencing to further boost ammoniogenesis from urea (fertilizing compound) including straws (e. g. paddy straw), stovers (e. g. corn) and legume haulms (e. g. vines). It is predicted that both convenience and sustainability will win the battle between chemical and biological approaches in feed pretreatment of this kind.
D) Particularly in certain "Cara-ranching" facilities in the Philippines there is the availability in certain regions for sugarcane tops (SCT) & bagasse pithe, and paddy straw in addition to the aquatic plant species being considered including Azolla (algae) as basal rations can be mechanized for convenience and ensiled for quality storage for year-round feeding supporting productive activities like dairy feeding. One proven e.g. is water hyacinth and paddy straw for dairy with smaller subsistence farming practices.